Creative BioMart can use recombinant protein expression technology to construct MHC-antigen molecule complex expression vectors, and express and purify these proteins. This service is designed to assist antigen display in viral infection-related research and antigen immunogenicity studies in viral immunization.

Viral Infection and Immune Clearance

When a host is invaded by a virus, the virus invades the host's cells to survive and replicate. Once the virus has entered a host cell and infection has occurred, the cells of the immune system cannot "see" the virus. Antigen display of host cells and subsequent cells uses molecules called major histocompatibility complex class I (MHC class I) proteins to display protein fragments intracellularly. These peptide fragments will include protein fragments produced by the virus after the cell has been infected with the virus. This way allows these cells to display information about the virus's interior to other immune cells. This is at the heart of the host cell's use of immune clearance mechanisms in conjunction with immune system cells to kill infected cells. The host's immune response to the virus includes killing infected cells through cytotoxic cells or eliminating pathogens through molecules such as interferons/antibodies. The difference is that interferons affect viral replication in host cells, whereas antibodies bind to proteins in their coat (adhesion) early in infection. Usually, the virus will use the host cell to express its own molecules after invading the host, which gives the host cell the opportunity to complete cell killing in the MHC class I pathway-dependent manner. In many studies on viral infection and its immune clearance mechanism, the complexes formed by MHC class I molecules and expressed viral antigens play an important role.

Fig.1 Viral Detection by Innate Immunity. (Michael Carty et al., 2021)

Expression of MHC Complexes Associated with Viral Infection

Our recombinant protein expression experimental team has extensive manufacturing experience. We offer a choice of multiple expression systems to try to meet your individual needs. Our immunologists have extensive experience in viral infection research, and they will help your viral research run smoothly. This service aims to provide the construction of MHC-peptide complex expression vectors related to virus immunity, the expression and purification of recombinant proteins, etc. Our service tenet is to provide researchers with high-quality scientific research services and products. Through this service, researchers can quickly obtain expression vectors or recombinant proteins. These products will aid research for hypothesis testing in related studies and further studies of immune mechanisms.

Available Services

• Construction of Expression Vector

We have independent molecular biology and bioinformatics teams responsible for vector design and construction, and we will recommend suitable expression vectors and tags for you according to your research project.

• Protein Expression of and MHC-peptide Complex

Our platform can also perform recombinant protein expression according to the vector provided by the customer. We have a variety of mature production models for cell expression systems, including prokaryotic expression systems, yeast expression systems, insect cell expression systems (baculovirus), and mammalian cell expression systems.

• MHC-peptide Complex Purification

This service also includes the expression and purification of recombinant proteins. Our technology platform can realize the choice of a variety of recombinant protein purification methods such as tag-specific affinity purification, and our products can guarantee a yield of >1mg and a purity of about 85%.

If you are interested in our services, please contact us for more detailed information.

References

• Carty, Michael, Coralie Guy, and Andrew G. Bowie. "Detection of viral infections by innate immunity." Biochemical pharmacology 183 (2021): 114316.
• Croft, Nathan P., et al. "Most viral peptides displayed by class I MHC on infected cells are immunogenic." Proceedings of the National Academy of Sciences 116.8 (2019): 3112-3117.