Creative BioMart developed specialized staining protocols for CD4 + and CD8 + T cells to ensure highly specific staining of our tetramers for best results. Our professional MHC researchers are committed to developing tetramer detection and functional analysis combined services, generating multi-functional tetramer technology and designing customized solutions to meet the needs of customers for antigen-specific T cell research.

Introduction of MHC Tetramer Assays

Most T lymphocytes express clones and highly specific antigen receptors (TCRs) on the cell surface. TCR (T cell receptor) binds to MHC peptide complex and recognizes antigenic peptides presented in MHC molecular environment. Because TCR has low affinity for MHC peptide complex and fast dissociation rate, the recombinant soluble monomer MHC peptide complex has not been used to detect antigen-specific T cells.

Tetramer analysis is used to detect and quantify antigen-specific T cells. The interaction between peptide MHC complex (pMHC) and homologous T cell receptor (TCR) is too low to be detected in flow cytometry. The development of tetramer MHC peptide complexes has led to the direct visualization, quantification and phenotypic characterization of antigen-specific T cells, which greatly enhances our ability to evaluate cell dynamics during immune response. The multicomponent polymerization of monomeric biotinylated pMHC by streptomycin complexation increases the desire of pMHC streptomycin tetramer for homologous TCR in order to detect antigen-specific T cells.

Process of MHC Tetramer Staining

MHC tetramer technology is based on the ability of MHC peptide complexes to recognize antigen-specific T cells at the single-cell level. This breakthrough technology enables researchers to accurately measure targeted T cell responses in infectious diseases, cancer and autoimmune diseases.

Our tetramer staining service has the selection of class I and class II MHCs and is used in a variety of cells, including human cells and animal cells. The following are the service steps.

Fig.1 Process of MHC Tetramer Staining

Advantages of Creative BioMart

• Multiple product lines for MHC tetramer
• Distinct fluorochromes for different samples
• Flow cytometry analysis optimization
• Antibody libraries for teasing
• Widely used in various cells

Turnaround Time

2-3 weeks

Our comprehensive services range from early project design to final implementation, including intelligent analysis, polymer design, T cell detection to final report formation. The service cycle is about 2-3 weeks, and customized scheme for you to meet your time and experimental needs.

Creative BioMart have unique MHC tetramer provides many new insights into T cells, enabling the study of multiple functions of T cells. We can provide comprehensive T cell detection and customized combined tetramer for functional testing services. If you are interested in our services, please do not hesitate to contact us for more detailed information.