Creative BioMart has skilled and experienced research scientists in the field of MHC providing comprehensive results by ELISpot assays in combination with our diverse range of MHC antibodies and proteins.

Introduction of MHC ELISpot Testing Service

The enzyme-linked immunospot (ELISpot) assay is a commonly used method for monitoring immune responses. It can be used as an experiment related to drug development studies and the development of high quality and reliable ELISA data is essential for scientific understanding and further investigation. These assays are commonly used in adaptive immune response systems to monitor responses in different humans and animals, and it has been adapted to test cytokines secreted by T cells. Therefore, this experiment is also essential to understand the T cell response in MHC.

Therefore, ELISpot was used in MHC-related studies to detect CD8+ and CD4+ T cell responses and was performed by optimal peptide design, which would depend on the type of MHC epitope response being analyzed, targeting alleles to determine the length of the peptide sequence. This method has several advantages, such as being more sensitive than other techniques, as well as the production of data that provides more quantitative and qualitative results than other methods, such as intracellular MHC staining.

Our MHC ELISpot Testing Service

Our MHC ELISpot assay process consists of the following steps.

• Target MHC protein-specific encapsulated antibodies immobilized on solid phase (96-well plates)
• Addition of immune cells and the presence or absence of appropriate stimuli (positive controls), including MHC peptide antigens
• Capture of secreted cytokines by immobilized antibodies
• Antibody detection after washing to measure captured cytokines
• Specific detection of combinations of antibodies, couplers and precipitation substrates to visualize captured proteins

Our report will show each colored spot, which represents a cytokine-secreting cell. Spots can be counted by the naked eye or using an automated plate reader, and many different cytokine and MHC histotypes can be detected using this method. Usually, the size of the spot indicates the affinity for cell binding. The higher the affinity for T-cell recognition, the higher the productivity of producing large, well-defined spots.

Applications

ELISpot assays are used in many areas of research, such as cancer, infectious diseases, autoimmune diseases, allergies and organ transplants. In T-cell studies at MHC, it is mainly used for immune monitoring and epitope discovery.

• Immune monitoring

Our products and services can be seen in this T-cell immunization monitoring. We can implement T-cell target discovery including MHC and related peptide libraries with design for cellular and immunotherapeutic use.

• Epitope discovery

ELISpot is the key method used to identify new epitopes. Our services can be used to develop strategies to produce immunotherapies or vaccines for a wide range of disease areas such as cancer or infectious diseases by identifying new epitopes.

Advantages of Creative BioMart

• Comprehensive MHC protein and antibody support
• Experienced technical team for immunology research
• Personalized ELISpot solution design
• Efficient final report delivery

Creative BioMart offers high quality ELISpot assays, providing our customers with a wide range of analytical options. Based on our advanced detection systems, we provide biomedical services to clients seeking to quantify and detect antibody-antigen immune response interactions. If you are interested in our services, please do not hesitate to contact us for more detailed information.