Creative BioMart offers a range of analytical test services for the analysis of MHC binding to peptides. Our service platform helps researchers improve the safety and efficacy of new biologics when developing therapeutic biological proteins by generating reliable data that can be used for early assessment of immunogenic epitopes.

Connotations of MHC/peptide Binding Assays

Responses from T cells are increasingly recognized as a key consideration when designing new vaccines. The contribution of cellular responses, especially responses from CD8+ T cells, is increasingly recognized as a key consideration in the development of anti-infective vaccines and new cancer vaccines. Current vaccine development efforts focus on aiding the discovery and design of T-cell epitopes through in silico prediction platforms, the most widely used of which is the Immune Epitope Database (IEBD) and Analysis Resource. The epitope-recognition algorithm used by IEDB combines multiple aspects of MHC class-specific affinity, but affinity-based strategies have limitations. Immunogenic epitopes require stable MHC/epitope interactions. This is due to the constant association and dissociation of labile epitopes with MHC molecules in vivo. Affinity measurements may therefore identify false positives, leading to unreliable predictions of epitope immunogenicity. Such false positives should be identified early in vaccine development to optimize immunogenic epitopes and lead to higher vaccine efficacy.

Fig.1 Customizing a patient-specific cancer vaccine. (Ugur Sahin et al., 2018)

Available Services

Our MHC peptide complex assay platform provides the following services for MHC peptide binding analysis, including affinity analysis and stability testing. Comprehensive testing services are used to measure the affinity of putative epitopes for multiple HLA alleles. Our assays use custom synthetic peptides as references and are analyzed in combination with the following service options. Our refined classification enables us to more comprehensively meet customer needs and provide customers with personalized testing solutions. Combined with peptide binding assays and stability testing, Creative BioMart provides a comprehensive understanding of the potential immunogenicity of target molecules.

Service Items

• MHCI/Peptide Affinity Assay
  MHC Peptide Binding Assays for MHC Class I Alleles Uncover the best possible T cell epitopes with a powerful in vitro assay.
• MHCII/Peptide Affinity Assay
  MHC peptide binding assays targeting MHC class II alleles are used to determine target molecule immunogenicity, aiding the development of novel immunotherapies and therapeutic biological proteins.
• MHC/Peptide Stability Assay
  This testing service is used to assess the stability of complexes with MHC molecules and peptides, which is an aspect of immunogenicity assessment.
• MHC/Peptide Competition Binding Assays
  This service is designed to evaluate the MHC binding properties of test peptides compared to control peptides.
• Epitope Priority Analysis
  These services focus on a comprehensive analysis of results generated from affinity and stability measurements to better rank immunogenic epitopes.

Creative BioMart has the ability to perform comprehensive MHC-peptide complex binding experiments to help customers perform comprehensive immunogenicity assays. This service includes multiple choices of service options, refined classification, to meet the diverse needs of customers as much as possible. Our experimental platform enables high-quality analytical testing. If you are interested in our services, please contact us for cooperation.

References

• Sahin, Ugur, and Özlem Türeci. "Personalized vaccines for cancer immunotherapy." Science 359.6382 (2018): 1355-1360.
• Kim, Yohan, et al. "Immune epitope database analysis resource." Nucleic acids research 40.W1 (2012): W525-W530.