Creative BioMart has unique peptide microarrays on solid surfaces which are effective tools for biomarker discovery, immune monitoring, antibody epitope mapping, multiplex epitope mapping or detection and validation of protein interactions in MHC studies.

Introduction of Peptide Arrays for Epitope Mapping

Peptide arrays are collections of peptides displayed on a solid surface, usually have a slide or membrane. Peptide arrays with immobilized peptides are used to study the binding properties, function and kinetics of protein-protein interactions, a key technique for mapping antibody epitopes and finding protein-bound residues in studies related to MHC. Since the development of therapeutics relies on detailed knowledge of these structural domains, peptide libraries are used to display multiple short, linear peptide fragments and derive specific epitopes in parallel. The most common applications for epitope mapping are T cells and antibodies.

Peptide arrays show overlapping peptide scans by MHC delivery of antigen or any other peptide set. They can be incubated with antibodies, serum or other patient samples. For example, peptides from viral or bacterial antigens, overlapping peptides from tumor-associated antigens, collection of antigens from specific pathogens or cancer types, can all be useful for MHC-associated immunotherapy studies.

Workflow of Peptide Arrays for Epitope Mapping

Our epitope mapping is the process of identifying the specific peptide sequence on an antigen that triggers an immune response by binding to an antibody or MHC molecule. Epitope identification requires systematic screening of antigens, which can be difficult when antigens have multiple conformations or binding structural domains.

• Our basic workflow

Our peptide arrays for epitope mapping service are designed by our scientists according to the corresponding workflow. Our workflow has the basis of many years of experience and optimized to the fullest in order to obtain the best possible results. The processes used for the project are listed below.



• Our epitope mapping steps

Our epitope mapping platform in which peptide sequences are synthesized directly into the immobilized matrix generates highly sensitive arrays that can reliably detect even the weakest binding signals. These immobilized peptide arrays are reusable and can be tested multiple times. Below are the steps of our epitope analysis.

Advantages of Creative BioMart

• Experienced epitope analysis scientists
• Broad applicability of MHC's multiple molecules
• Testable targets and sample diversity
• High sensitivity of epitope mapping platform
• Reusable peptide arrays

Turnaround Time

5 - 12 weeks

Our service is highly efficient with turnaround times of 5-12 weeks. It will provide you with a complete report of MHC epitope mapping, which will be very useful for your research on vaccines for many diseases and cancers.

Creative BioMart has years of experience in immunology and biochemistry, our epitope mapping based on MHC-associated peptides provides accurate, clear and directly applicable epitope definitions for the samples you want to test. We offer you a unique platform that allows us to define different epitopes accurately and cost-effectively. If you are interested in our services, please contact us for more detailed information.