Creative BioMart offers the combination of MHC alleles and peptide pools, highlighting T cell reactivity in antigen target discovery of samples. Because the frequency of any particular MHC allele can be elucidated, this type of analysis is expected to be a valuable method for estimating the likelihood of T cell responses within a group.

Introduction of Peptide Pool for Antigen Target Discovery

The peptide library contains a variety of restricted epitopes of MHC class I or class II molecules from human viruses. Most patients who have been exposed to these antigens and possess the corresponding CD8+ and CD4+ memory T cells. This means that, unlike conventional reagents, the MHC-associated peptide pool is well suited for use as a specific positive control for all T cell-based assays, such as intracellular flow cytometry analysis or ELISPOT assays.

T cell activation assays rely on donors that are exposed to specific proteins or peptides for uptake by antigen presenting cells (APCs) and eventual presentation of MHC-treated peptides to T cells. Several methods can be used to analyze the T cell adaptive immune response against the protein. Some of these assays include ELISPOT for the detection of secreted cytokines and immunostaining for intracellular cytokines and markers of cell membrane activation.

Our Peptide Pool for Antigen Target Discovery

We used synthetic overlapping peptide libraries with multiple sequences for T cell activation assays. Each library consists of several short peptides with overlapping amino acids. After incubation of the donor source with each peptide library, T cell activation and target discovery were determined based on positive immunostaining.

Our services have a complete process, from the start of design to the final delivery of results. Unknown antigens and epitopes are discovered through optional antibody specificity assessment. Project selectable samples include monoclonal or polyclonal antibodies, hybridoma supernatants, purified antibody fractions, serum, plasma, etc.

Our peptide pool service contents include the following, as well as meeting your other individual customization requirements.

• MHC-associated peptide synthesis
• MHC-associated peptide pool design
• MHC-associated peptide matrix design

Applications

• Antigen-specific stimulation of T cells

Identifying and discovering the best antigens for your research.

• Cellular immune response analysis

Immune monitoring during disease or treatment.

• Vaccine efficacy assessment

Identifying antigens for vaccine target testing

Advantages of Creative BioMart

• Reliable biochemical control and quality assurance of MHC-associated peptide pool generation
• Covering all MHC epitopes
• Risk control for toxic suppression of T cell responses
• Rapid peptide pool production and report analysis
• The most affordable custom peptide pool

Turnaround Time

Our service time depends on the number of your target MHC genes and custom synthesized peptides. And we are sure to present the fastest and best results. As a result, we are able to provide a pool of MHC-associated peptides very quickly and cost-effectively. The MHC-associated peptide pool is optimal for a wide range of antigen target discovery, T-cell epitope discovery and biological screening.

Creative BioMart has also used it for a variety of T-cell assays for immune monitoring. Our antigen discovery pools consist of overlapping and hybrid peptides synthesized with our high-throughput technology, fully automated and purified by dialysis. If you are interested in our services, please contact us for more detailed information.