Creative BioMart offers a variety of MHC protein modification protocols, such as protein phosphorylation, protein methylation or protein acetylation, providing an important tool for creating therapeutic conjugates and generating novel protein constructs, and usually the restriction epitope of MHC is closely related to the development of cancer vaccines. We have extensive experience in protein chemistry modification and are confident in providing the best protein modification services to meet the specific needs of our customers.

Introduction of MHC Protein Modification

There is growing evidence that inflammation, transformation, apoptosis, and aging can alter modifications. Modifications associated with the MHC protein leading to alterations in the library of associated peptides, which may be important in the immune response associated with autoimmune diseases, infections, and cancer.

And there are a variety of different modifications offered by the types of Class I and Class II MHC molecules. Some modifications promote the binding of peptides to MHC molecules and may also affect the ability of antigen processing pathways to produce peptides.

Process of MHC Protein Modification

We can provide MHC protein modification and analytical reports for subsequent studies. Our MHC protein modification mainly includes the following steps, as well as the detection experiments and reports of the modification afterwards, from T cell clones and primed lines, peptide library scan, expression plasmids to Protein expression, refolding, and purification.

Fig.1 Process of MHC Protein Modification.

Our Protein Modification Services

• Protein phosphorylation

The phosphorylation of the invariant chain by protein kinase regulates MHC molecule trafficking to antigen-processing compartments, our modification can make contribute to your research.

• Protein methylation

Methylation and expression of MHC class I and class II affect in human developmental tumor cells and in T cell malignancies, related modification can be offered with professional operation.

• Protein acetylation

Acetylation regulates the MHC class II expression and antigen presentation in tumor cells, which is possible achieved in immunology experiments.

• Post translational modification

Different post-translational modifications promote the binding of peptides to MHC molecules, and might also influence the ability of the peptide to be produced by antigen processing pathways. 

• Customer-tailored service

If you have other modification requirements, we can provide optimized scenarios to assist your scientific research with our years' experience in advanced experiment equipment. You can choose online inquiry to describe the project requirements or contact us for consulting the direct information.

Advantages of Creative BioMart

• Customized modification scheme design
• Multiple cell lines
• Abundant peptide library
• Complete analysis and report

Applications

• Vaccine design

Our MHC modification service improving T cell Ags by altering MHC anchor residues as a common strategy can be used to enhance peptide vaccines.

• Immune interventions

Vaccination with heterologous peptides may trigger T cells, so our various MHC protein modification related to immune interventions are required to detect evaluation to ensure clinical efficacy.

• Cancer

By recognizing short peptide fragments that are compounded with cell surface MHC molecules, our modifications operated in T cells coordinate adaptive immune responses and provide protection against pathogens and cellular malignancies

• Agonist improvement

The replacement of MHC I can be offered in our service binding sites with suboptimal anchoring residues produces significantly which improved T cell agonist peptides.

Turnaround Time

4-8 weeks

Our service is highly efficient with turnaround times of 4-8 weeks. We have a high degree of expertise in protein engineering while offering a variety of protein modification methods and the most appropriate modification analysis strategies.

Creative BioMart offers complete surface analysis, MHC stability assays, cellular assays and further study on flow cytometric sorting and molecular analysis of CD8+ T cell clonotypes on the basis of protein modification. For more detailed detection, we have developed a variety of methods to check the stability of MHC after measuring modification. If you are interested in our services, please contact us for more detailed information.

Reference

• David K, et al. (2010). Modification of MHC Anchor Residues Generates Heteroclitic Peptides That Alter TCR Binding and T Cell Recognition. The Journal of Immunology. 185 (4) 2600-2610.