Creative BioMart offers a unique T-cell stimulation assay that has wide application in research MHC studies. Because of the extensive experience we offer, our standard assays can often be easily adapted to your custom requirements.

Introduction of T Cell Stimulation

Danger signals from pathogens induce cells to enter maturation in a development that will transform the program into efficient antigen-presenting cells and T-cell activators by stimulation. Bacteria and viruses, as well as inflammatory cytokines and other self-molecules, induce cells through specific interactions. T lymphocytes, through CD4-dependent independent pathways, as well as through direct cell-to-cell contact and cytokine secretion, promote the final cellular response. Upon encountering danger signals, antigen uptake, intracellular transport and degradation, and the frequency of intracellular interactions of MHC molecules were altered. peptide load transport of MHC molecules to the cell surface were increased. surface expression of T cells was also elevated.

It has been demonstrated that the extracellular specificity of T cells increases when purified complexes of MHC-like molecules and antigenic peptides interact with the T cell receptor (TCR) on clonal T cells. Experimental studies have shown that this measure of increased specificity can be used to assess the various functional roles of MHC-like antigens. When equal amounts of modified MHC-related peptide complexes were exposed to restricted cloned T cells, an increase in the frequency of extracellular changes in T cells was observed.

Our T Cell Stimulation

Our service is based on antigen-specific T cell stimulation allowing stimulation of a strictly defined set of T cells, for example using tumor or MHC-associated peptides. These are processed by highly efficient antigen-presenting cells to produce the corresponding surface MHC that present the antigen and are then used to trigger T cell activation.

The mixed lymphocyte response (MLR) is commonly used in our service and is based on the principle of determining the degree of compatibility of the recipient and donor major histocompatibility antigens (HLA antigens). MLR is often used as a means to induce general stimulation/activation of T cells in culture. We can measure the MLR to determine the immunomodulatory power of the active compound, which is a useful method, and the MLR has the advantage of not requiring any other stimulating compound is.

Researchers can turn to us for help in designing custom experiments, and we offer the following service components.

• Assessing immune regulation
• Characterization of the response to antigen
• Testing for immunogenicity risk
• Immunological monitoring
• Immunogen sample comparison

Advantages of Creative BioMart

• Strong baseline response to T-cell stimulation
• A wide range of flow cytometry assays
• Ability to analyze large samples
• Multiple T cell donor offerings

Creative BioMart will provide you with the most suitable T-cell stimulation protocols and can design custom experiments for researchers to come. Due to our extensive experience, our T-cell standard assays can often be easily adapted to your custom requirements. Our team of scientists are expert immunologists and can understand your needs in detail. If you are interested in our services, please do not hesitate to contact us for more detailed information.