Creative BioMart provides recombinant protein expression vector construction and expression cell line construction services. Our Molecular Editing Service is able to handle the expression service of peptide-MHC molecules relevant for in-house antigen presentation. This service is designed to help clients express antigen presentation responses associated with CD8+ T cells.

CD8+ T Cell-associated Immune Response

Major histocompatibility complex class I (MHC I) is required for viral antigen presentation and subsequent killing of infected cells by cytotoxic T lymphocytes. A key determinant of CD8+ T cell recognition of virus-infected cells is the binding of the T cell receptor (TCR) to its cognate peptide/MHC I complex. When MHC I is transported through the endoplasmic reticulum (ER), short peptides of 7-13 amino acids in length from the host or foreign proteins are loaded onto MHC I and then presented on the cell surface. Therefore, many viruses such as HIV and herpesviruses have evolved complex mechanisms to interfere with MHC I presentation of viral peptides due to the critical role of MHC class I molecules in the recognition of infected cells by CD8+ T cells. MHC class I molecules play an important role in the study of the transport mechanism of endogenous antigens and the function of CD8+ T cells, helping researchers to accelerate the discovery of new targets.

Fig.1 Intracellular assembly of MHC I peptide complex. (Adiko et al., 2015)

Expression of MHC Complexes Associated with Intracellular Antigens

This service is a custom service for peptide-MHC I complex against intracellular antigens. Our recombinant protein production platform can produce pMHC monomers of target antigens according to the allele type, peptide sequence, fluorescent label, and other information provided by customers. At the same time, we also provide customers with MHC I multimer generation services, such as typical MHC I tetramers. Our custom monomeric and tetrameric reagents can aid in the study and analysis of CD8+ T cell function. In addition, our multimer reagents have good binding properties and stain cells well. And we also provide some reversible tags that can be added to complex monomers or polymers for easy analysis by researchers.

Advantages of Creative BioMart

• We provide one-stop services from gene synthesis and vector construction to protein expression and purification, and can select different expression/purification tags and expression hosts according to customer needs, so as to truly achieve deep and private customization for customers.
• With a variety of purification systems, providing a variety of options for protein expression and purification, we are committed to providing customers with high-quality, low-cost recombinant proteins.

Creative BioMart's custom pMHC complex service provides a convenient tool for the analysis of target CD8+ T cell populations for intracellular antigen presentation. Our custom MHC complex products come in many forms, monomers, dimers, or tetramers. Likewise, we offer our customers reversible and irreversible labeling methods to provide the optimal MHC complex solution for your project. If you are interested in this service, please contact us for cooperation.

Reference

• Adiko, Aimé Cézaire, et al. "Intracellular transport routes for MHC I and their relevance for antigen cross-presentation." Frontiers in immunology 6 (2015): 335.