Creative BioMart provides MHC knockdown cell line construction services based on RNA interference technology, which can be used to study the role of MHC signaling and test the complex role of MHC molecular signaling in immune-related signaling. Our molecular biology team provides comprehensive interference strategy design and subsequent cell line evaluation, which can help researchers obtain more useful molecular and cellular tools.

MHC Signaling and Tissue Rejection

Major histocompatibility complex (MHC) molecules are known for their role in antigen (cross) presentation and thus play a key role in communication between immune cells. However, MHC molecules can also act as signal receptors and are involved in what is known as reverse MHC signaling, and the linkage of MHC molecules can lead to signal transduction and cellular regulation in antigen-presenting cells. In the case of MHC-I, for example, reverse signaling can produce a variety of outcomes, including apoptosis, migration, induction or reduction of proliferation, and cytotoxicity to target cells. MHC-I stimulates signaling pathways and signaling molecules such as Akt1, extracellular signal-regulated kinase 1/2 (ERK1/2) and nuclear factor-κB (NF-κB) in various immune and non-immune cells. Common signaling molecules activated upon MHC-I ligation in a variety of cell types encompass these molecules. Natural MHC-I ligands and extended downstream pathways are not fully understood, but reverse MHC-I signaling appears to be involved in regulating defenses against bacterial and viral infections and malignancies. The study of MHC signaling is therefore important for understanding its impact on disease physiology.

Fig.1 The antigen presenting role of MHC class I molecules and their structure. (Elke M. Muntjewerff et al., 2020)

Interfering Plasmid Construction and Cell Line Evaluation Service

Our MHC knockdown cell line construction service can be used to low-express MHC molecules in cell lines and further test their function. The key to this service is the silencing of regions associated with MHC molecules by RNAi technology. Our molecular biology team provides testing from interference plasmid design to cell line evaluation. Customers can specify the MHC gene to be knocked out or upload the designed siRNA or shRNA, of course, you can also fully entrust us to design. Our interfering fragments will be strictly quality controlled and used for subsequent low-expressing cell line construction.

Fig.2 The core of MHC knockdown cell line customization services.

Creative BioMart has molecular editing capabilities to help customers develop usable MHC knockdown cell lines. Our molecular biology team will provide you with the design of custom RNA interference strategies and molecular tools, or you can choose to upload sequences directly. We have accumulated rich experience in cell line construction, and have complete molecular platforms and testing services to meet the different research needs of our customers and help them maximize the success rate of their research projects. If you are interested in our services, please contact us for more detailed information.

Reference

• Muntjewerff, Elke M., et al. "Reverse Signaling by MHC-I Molecules in Immune and Non-Immune Cell Types." Frontiers in immunology 11 (2020): 3264.