Being able to accurately assess T-cell antigen-specific immune responses in immunological studies is essential for the development of safe and effective biological therapies and vaccines. Intracellular processing of protein antigens and induction of T cells via major histocompatibility complexes (MHC) are necessary steps in the immune response. This is why it is important to be able to provide a stable and diverse T-cell assays.

Creative BioMart offers a complete portfolio of T-cell assay services for a wide range of MHC research-related applications including T-cell target identification, T-cell epitope discovery and epitope mapping, and antigen discovery.

What Do We Offer?

• MHC ELISpot Testing Service

The ELISpot assay is a frequently used method for monitoring immune responses in MHC-related studies, detecting CD8+ and CD4+ T cell responses. It can be used as a relevant assay for drug development studies, and likewise this assay is essential for understanding T cell responses in the MHC. We analyzed the type of MHC epitope response by MHC optimal peptide design, targeting alleles to determine the length of peptide sequences, providing richer quantitative and qualitative results.

We offer the entire process of seeking to quantify and detect antibody-antigen immune response interactions with high quality ELISpot assay and wide range of analytical options.

• Flow Cytometry for Immune Monitoring

We have a flow platform that can directly measure antigen-specific T cells and monitor antigen-specific T cell responses. We use MHC-related peptides or antigens to stimulate antigen-specific T cells in order to analyze proliferation and cytokine production. This approach allows co-staining of T cell lineage markers and possibly other surface or intracellular markers, thereby increasing the level of obtained responses. The combination of flow cytometry and MHC protein staining allows measurement of cytokine expression and provides additional information about the immune response.

We offer analysis of cell generation by flow cytometry factors including but not limited to effectors and regulatory cytokines in response to stimulation by MHC-associated peptides, antigens or cell lines.

• MHC Tetramer Staining for T-Cell Analysis

MHC tetramers are often used to detect and quantify antigen-specific T cells. T cell receptor binds to the MHC peptide complex and recognizes the antigenic peptide presented in the MHC molecular environment. The interaction between the peptide MHC complex and the homologous T-cell receptor is too low to be detected in flow cytometry. The advent of tetrameric MHC peptide complexes allows for direct visualization, quantification and phenotypic characterization of antigen-specific T cells due to the low affinity and rapid dissociation of TCRs for MHC peptide complexes. This approach greatly enhances our ability to assess cellular dynamics during the immune response.

We offer MHC tetramer staining service that has the selection of class I and class II MHCs and used in a variety of cells, including human cells and animal cells, to accurately measure targeted T cell responses.

• T Cell Stimulation

Purified complexes of MHC-like molecules and antigenic peptides interact with T cell receptors on cloned T cells when T cells are stimulated. And the increased load of peptides transported by MHC molecules to the cell surface further aggravates the expression of surface molecules on T cells. T lymphocytes contribute to the final cellular response through CD-dependent independent pathways as well as through direct cell-to-cell contacts and cytokine secretion. This specific increase in T cell expression can be used to assess various functional roles of MHC-like antigens.

We offer antigen-specific T cell stimulation to produce the corresponding surface MHC that present the antigen and are then used to trigger T cell activation by MLR.

• T Cell Receptor Analysis

Receptors on T cells are limited to sensing the presence of protein antigens by binding to short peptides in antigens bound to major histocompatibility complex (MHC) molecules on the surface of other cells. There is research evidence that rapid shedding rates and sequential engagement of MHC peptide and TCR complexes may be critical for T cell activation, and tetramerized MHC class I and II complexes have been widely used to quantify and characterize antigen-specific T cells and to probe TCR-MHC interactions. The accessibility of various MHC peptide tetramers is very helpful for specific TCR studies.

We offer a wide variety of TCRs through gene rearrangement and somatic cell proliferation mechanisms that can respond to different antigens, determined by next-generation TCR library analysis.

• T Cell Epitope Mapping

Epitope mapping of isolated T cells can determine the precise location, number and size of T cell epitopes, which can be used to aid in de-immunization and help reduce the risk of clinical immunogenicity. We provide T cell epitope mapping services to researchers to help them uncover more potential knowledge and accelerate the development of new therapies and drugs.

• T Cell Function Test

The interaction of MHC and TCR generates a T cell-mediated immune response, and MHC-associated molecules present the peptide to antigen-specific T cells that recognize the peptide. Use MHC molecules to discover antigen-specific T cells as well as to assess T cell-specific functions. Our MHC-related products offer enhanced T-cell assay specificity and flexibility, enabling researchers to rapidly assess the regulation of T-cell function by novel targets through our range of T-cell assay services.

Advantages of Creative BioMart

• A new generation of analytical technology
• Personalized ELISpot solution design
• Flow cytometry analysis optimization
• Strong baseline response to T-cell stimulation
• Multiple product lines for MHC tetramer

Creative BioMart has customized T-cell testing services that include T cell stimulation, T cell receptor analysis and MHC tetramer staining by multiple testing tools, such as ELISpot testing and flow cytometry. If you are interested in our services, please do not hesitate to contact us for more detailed information.