Creative BioMart custom DNA-Barcoded pMHC Multimer services based on DNA barcoding technology. This method, which can be applied to the high-throughput analysis, can improve the ability of T-cell profiling. We will help you design DNA barcodes, and complete the production and labeling process of the pMHC Multimer. By choosing our custom service, you provide products that can be used directly for analysis.

DNA Barcode-labeled MHC Multimer

The advent of MHC polymer technology has enabled breakthroughs in the quantification and analysis of antigen-specific T cells. In particular, this technology has greatly advanced the measurement and analysis of CD8 T cells, expanding the range of applicable cells, such as CD4 T cells, natural killer T cells, mucosa-associated T cells, etc. MHC multimeric complex tools for detection Antigen recognition-based T cells can link multiple tags, and DNA barcoding is a unique DNA barcoding technology. When attached to an MHC multimer, it forms a specific tag for a given pMHC epitope. DNA barcodes are designed with sequence complexity, with each sequence forming a unique tag. Therefore, this strategy is not limited by the number of tags available, which has previously been the most critical hurdle for high-throughput detection of antigen-reactive T cells. It has been reported that the method can be used to screen more than 1000 specificities in a single sample.

Fig.1 Detection of antigen-responsive T cells using the DNA barcode-labeled MHC multimer methodology. (Bentzen, Amalie Kai et al., 2017)

DNA-Barcoded pMHC Multimer Customization

Our platform provides DNA barcode-based customization of pMHC multimers. This service is based on the production of MHC multimers, and the pMHC multimers are labeled by DNA barcoding technology. Compared to fluorescent-based labels, this label enables high-throughput screening. We tandem biotinylated molecules, recombinant MHC, and DNA barcodes through a polysaccharide polymer backbone with numerous free streptavidin (SA) binding sites. Each pMHC epitope is associated with a unique DNA barcode that can be read by PCR or sequencing. Barcode tag screening enables researchers to correlate reads of specific DNA barcodes matching the corresponding pMHC with antigen-reactive T cells in the sorted population.

Advantages of DNA-Barcoded Technology

DNA barcoding technology has the characteristics of high-throughput analysis, which expands the analysis potential of pMHC multimers.

The way DNA barcodes can be read by PCR or sequencing.

DNA barcoding of MHC multimers provides a new tool to gain insights into T cell recognition in complex disease situations.

Fig.2 DNA barcoding expands the capabilities of MHC polymer technology.

Creative BioMart's protein production platform has the ability to generate pMHC multimers, and we provide customers with DNA-Barcoded pMHC Multimer customization services on this basis. This tag has unique advantages compared to fluorescent labels, enabling high-throughput T cell analysis and expanding the ability of MHC multimer technology to analyze T cells. If you are interested in our services, please contact us for more information.

References

• Bentzen, Amalie Kai, and Sine Reker Hadrup. "Evolution of MHC-based technologies used for detection of antigen-responsive T cells." Cancer immunology, immunotherapy 66.5 (2017): 657-666.
• Dahotre, Shreyas N., et al. "DNA-barcoded pMHC tetramers for detection of single antigen-specific T cells by digital PCR." Analytical chemistry 91.4 (2019): 2695-2700.