Creative BioMart offers custom services for MHC-overexpressing cell line construction and its lysates. This service enables researchers to obtain cell lysates with high expression of MHC molecules to test the role of MHC molecules in related diseases. Our molecular biology team provides the entire process from strategy design to obtaining MHC-expressing cell lysates.

MHC Over-expression and Disease

Folding and transport of proteins are tightly regulated in all cells through the endoplasmic reticulum, such as major histocompatibility complex (MHC) class I. MHC overexpression plays an important role in the pathophysiology of some diseases. Taking protein-rich muscle tissue as an example, overexpression of MHC class I proteins, a common feature of many myopathies, including idiopathic myositis, induces ER stress. Studies have investigated the consequences of MHC overexpression in muscle at different ages. Using a transgenic model of myositis induced by MHC class I protein overexpression, it found that the younger group had a more severe disease phenotype, with rapid onset of muscle weakness and pathology. Of course, researchers can also use in vitro overexpression constructs, and the obtained custom proteins can be used to study the pathological or physiological changes caused by MHC overexpression.

Fig.1 Forced expression of H-2Kb induced ER stress. (Manuel Fréret et al., 2013)

Construction of MHC Over-expressing Cell Line

Overexpressing cell lines have been widely used in biological research, such as protein engineering, recombinant antibody development, drug discovery, etc. Your gene of interest will be integrated into the host cell after transduction and be permanently passed on to the next generation of cells and express some protein of interest. We provide MHC-overexpressing cell lysate preparation services, which will help customers study the effects of MHC class I and class II overexpression on disease. Cell lysates can be used for subsequent analytical testing by constructing overexpression cell lines and harvesting cell lysates. Our gene-editing platform offers a variety of vectors for overexpression, including viral vectors (lentivirus, adenovirus) and plasmids. Transduction with a designed expression vector provides a stable expression of the gene of interest (GOI), our advantage is the use of lentivirus to infect cells. Compared to plasmids, lentiviruses have broad tropism and can target most mammalian species and cell types. Our molecular biology team provides services ranging from strategy design, suitable vector construction, viral packaging, and viral transduction to positive clone selection. At the same time, we also accept the construction plan submitted by customers, and we will complete the next steps for you. If you have a specific product requirement, Creative BioMart Custom Stable Cell Line Services goes to great lengths to meet all of your needs.

Fig 2. Overexpression cell line construction and cell lysate acquisition.

Creative BioMart offers customized cell line services to provide researchers with MHC-overexpressing cell lines or their lysates. Our molecular team has extensive experience in the customization of genetically modified cell lines to meet the diverse research needs of our clients. If you are interested in our services, please contact us for more detailed information.

Reference

• Fréret, Manuel, et al. "Overexpression of MHC class I in muscle of lymphocyte-deficient mice causes a severe myopathy with induction of the unfolded protein response." The American Journal of Pathology 183.3 (2013): 893-904.