MHC-associated peptide sequences are presented by the major histocompatibility complex (MHC) on antigen-presenting cells. Therefore, they may induce immunogenicity. Activation of lymphocytes in tumor cells through the binding of their epitopes to antigen receptors on the surface of the corresponding lymphocytes elicits an immune response, and MHC-associated peptides have been studied by predicting the appropriate selection of epitopes to control their immunogenicity.

Creative BioMart is a peptide expert in MHC-associated peptide research, and our scientists have many years of experience. We are known for our innovative services for high-quality MHC-associated peptides, including synthesis and modification, isolation and analysis as well as proteomic analysis and peptide design of MHC-associated peptides for epitope and antigen discovery.

What Do We Offer?

• MHC-associated Peptide Synthesis

MHC molecules present thousands of peptides on the cell surface. These MHC-associated peptides, collectively known as the immunopeptidome, regulate every step of T-cell development and function. MHC alleles are highly polymorphic and each homozygote has a specific peptide-binding motif that allows for the synthesis of MHC-associated peptides by sensitizing target cells and thus screen for epitopes recognized by T cells.

We offer high quality custom peptide synthesis services by various synthesis methods, such as solid phase peptide synthesis (SPPS), liquid phase peptide synthesis (LPPS), which is analyzed by LC-MS, MALDI-MS or HPLC to confirm the identity and quality of your custom peptide.

• MHC-associated Peptide Modification

Experiments have shown that modification of MHC-associated peptides affect their presentation to T cells, with acetylation at the N-terminal end and amidation at the C-terminal end enhancing the ability of the peptide to activate T cells. Simultaneous modifications further increased the stimulatory activity, with the peptide becoming several times stronger than the unmodified peptide.

We offer the most appropriate methods and techniques for your MHC-associated peptide project, our custom peptide modifications include N-terminal, C-terminal, non-standard residues and other modifications.

• Isolation and Analysis of MHC-associated Peptides

During T cell-mediated cancer immunotherapy, the T cell epitopes contained in these tumor proteins determine the effectiveness of the therapy. With the establishment of methods for the isolation and analysis of MHC-associated peptides, peptides that can be eluted from MHC peptides have been sequenced, and many epitopes have been identified that have important research implications for immunotherapy.

We offer suitable methods as solutions for the isolation and analysis of MHC-associated peptides with our advanced instruments, fit-for-purpose labs and expertise.

• MHC-associated Peptide Arrays for Epitope Mapping

Rational vaccine development and evaluation require identification and measurement of the size of epitope-specific T cell responses and epitope mapping. This process can be accelerated in research by using ultra-high density of MHC-associated peptide arrays as a high-throughput tool for screening peptides to identify putative new epitopes.

We offer peptide arrays for epitope mapping service designed by our scientists with many years of experience, and highly sensitive arrays can reliably detect even the weakest binding signals.

• MHC-associated Peptide Pool for Antigen Target Discovery

The MHC-associated peptide pool is widely applicable to many disease areas, including all areas of cancer and infectious diseases. The T cell epitopes identified by this technology can be used to assess protein antigenicity or as core building blocks for vaccine development or as targets for new immunotherapies.

We offer a complete process, from the start of design to the final delivery of results. Unknown antigens and epitopes are discovered through optional antibody specificity assessment.

• MHC-associated Peptide Library Design

Peptide library contains a large number of peptide sequences used to determine the function or binding of key fragments. Peptide libraries are used for a wide range of applications such as proteomics, vaccine development, epitope mapping, or cancer therapy. The selection of the appropriate MHC-associated peptide length and offset number depends on the application of the peptide.

We offer overlapping peptide library, truncation library and random library, providing you with the possibility to screen for modified peptides containing unnatural type amino acids for your MHC- associated peptides.

• MHC-associated Peptide Proteomics

The analysis of immunogenicity-related peptides presented on the MHC, known as MHC-associated peptide proteomics (MAPP), can be applied to search for antigens presented on the MHC for drug development. The use of LC/MS to study the composition and kinetics of peptides presented by MHC molecules is important for the discovery of T cell targets to combat cancer as well as epidemic pathogens.

We offer MHC-associated peptide proteomics assays that use powerful MS analysis combined with in vitro T-cell assays to identify epitopes that trigger T-cell responses.

Advantages of Creative BioMart

• MHC-associated peptide synthesis specialist
• Wide range of modifications for MHC-associated peptide
• Personalized MHC-associated peptide library
• Fast delivery and quality assurance

Creative BioMart can synthesize and analyze your MHC-associated peptides. With our state-of-the-art peptide research platform, we can provide assistance in proteomics, epitope mapping, cancer therapy, and therapeutic antibody development for a variety of applications. If you have other requirements, we can customize the solutions for you. Please contact us for telling your project interests.