Creative BioMart is a provider of immune-related proteomics services for the quantification and screening of MHC-binding peptides involved in immune response initiation. We feature mass spectrometry combined with multivariate analysis to identify peptides recognized by MHC molecules in immune abnormalities caused by specific drug stimuli, disease or infection.

MHC-associated Peptide Screening and Quantification Assay

The MHC Class I and II Molecularly Associated Peptide Identification Service is designed to assess the size of the fragments bound by MHC based on the adequacy of the isolation procedure and the parameters used for neoantigen detection. Quantitative proteomics is an effective method to understand protein dynamics in cells, tissues, or organisms. Mass spectrometry-based peptidomics analysis methods are widely used in the development of immune-related biomarkers and the analysis of peptide molecules. Currently, mass spectrometry-related techniques widely used in peptidomics analysis include MS, LC-MS, LC-MS/MS, etc. Through these techniques, researchers can get a glimpse of what the MHC-bound peptide looks like. Peptide-MHC (pMHC) complexes from biological samples often have complex components, and affinity purification can enrich MHC (or a class of them) in the sample. The MHC-bound peptides are isolated by elution, which can then be further analyzed and their structures described.

Our Peptide Screening and Quantification Service

The choice of this testing service will depend on the size and type of sample, available budget, and of course your goals. The choice of this testing service will depend on the size and type of sample, available budget, and of course your goals. We provide peptide-MHC complex purification and quantitative analysis and detection services based on HPLC-MS or HPLC-UV, and also accept mass spectrometry analysis and detection of customer purified samples. We select high-quality peptide libraries for targeted proteomics and reference peptides for targeted proteomics to complete the test. We can understand your options and recommend the best workflow for your experiment.

Our services of MHC quantification work include workflows for all of these processes.

• Purification of peptides of peptide-MHC complexes by HPLC;
• Mass spectrometry identification of purified peptides;
• Customized MHC- associated peptide libraries for immunopeptideomics;
• Protein post-translational modification analysis;
• Effective quality control methods;

Advantages of Creative BioMart

• Our service provides optional fit purification steps, and customers can choose to provide lysates or purified peptides.
• Our MHC-related peptide scanning and quantification service is a comprehensive service that also enables excellent detection and quantification of targeted peptidomics;
• Our testing services are regulated and modified with high quality and will provide you with high quality analytical results.

Creative BioMart has a variety of peptide quantification methods that overcome the limitations of traditional methods, such as limited accuracy and high cost. Our scientists can perform reliable and reproducible MHC and associated peptide quantification with HPLC/MS. If you are interested in our services, please do not hesitate to contact us for more details.