Creative BioMart can reconstruct several MHC molecules of multiple strains into peptide-filled and peptide-deficient forms, and purify these proteins for biochemical studies. The recombinant MHC proteins we can express include fusion proteins, cell antigens, and human β-2-microglobulin (β2m), both in wild-type and mutant variants.

Introduction of MHC Proteins

Major histocompatibility complexes (MHCs) are a family of genes responsible for many key functions of the immune system of most vertebrates. MHC consists of three classes that are distinguished according to their structure and function. Purified MHC molecules can be used to study their interactions with transmembrane chaperones and helper proteins residing in the endoplasmic reticulum to better understand class I antigen presentation pathways.

MHC monomers can be used in interactions with peptide complexes with ligands such as monoclonal antibodies, recombinant T cell receptors or other binding scaffolds, for example for screening new antigen-specific CAR T cell antigen receptors.

Fig.1 Difference between two kinds of MHC proteins. (Kenneth L. Rock, 2016)

Our Recombinant MHC Protein Expression Process

Manufactured by an experienced team that offers flexible labeling options, the widest range of catalog items, long-term storage, short-time delivery and stable detection of antigen-specific T cells, and our MHC proteins are the industry leader in understanding how your immunization works.

1) Different MHC species transformation.

We offer MHC recombinant proteins of different species including human, mouse, monkey, etc. We have different MHC expression system offered by your scientific needs, including cell expression system, cell-free expression system, bacterial expression system, yeast expression system, mammalian cell expression system and other system designed for your solutions.

2) Large-scale MHC I and II protein production.

Production of a wide range of MHC Class I and Class II will be available in the form you want with or without related peptide. Our large-scale expression system is suitable for different MHC-related research, such as associated proteome and structural biology and applicated in industrial-scale production.

3) Multiple chromatography methods.

We have affinity Chromatography, ion-Exchange Chromatography, size-exclusion Chromatography, proteolytic Cleavage and other purification steps to provide you with well homogenized MHC recombinant proteins. We have established various purification strategies for different kinds of MHC protein with our skillful and experienced scientists.

4) Different modifications.

MHC proteins are subsequently modified with various modifications such as methylation, acetylation, and acetylation to produce proteins suitable for scientific research. The purification of MHC protein will be achieved as soon as possible to save your time.

Table 1. Protein Selections

Advantages of Creative BioMart

We provide one-stop services from gene synthesis and vector construction to protein expression and purification, and can select different expression/purification tags and expression hosts according to customer needs, so as to truly achieve deep and private customization for customers. A variety of purification systems, providing a variety of options for protein expression and purification, we are committed to providing customers with high-quality, low-cost recombinant proteins.

• High efficiency expression vectors and cells
• Large scale protein expression and production capacity
• Rich experience in protein purification
• Various protein purification and refolding techniques
• Fast and economical

Turnaround Time

4-8 weeks

Creative BioMart has the ability to develop a variety of new proteins and have accumulated rich experience in the expression and production of recombinant proteins. We have a variety of recombinant protein expression systems, including insect cells, mammalian cells and prokaryotic protein expression systems, to meet the different research needs of our clients and help them maximize the success rate of their research projects. If you are interested in our services, please contact us for more detailed information.

References

• Cheng, Loi, et al.  "In Vitro Expression and Purification of Class I MHC Molecules" (2006). Honors Scholar Theses. 397.
• Kenneth L. Rock, et al. “Present Yourself! By MHC Class I and MHC Class II Molecules” (2016). Trends Immunol. 37(11): 724 –737.