Creative BioMart provides MHC-associated immunophenotyping, which is performed by incorporating our specialized flow cytometry staining experiments. Using our technical capabilities and saving time by placing data collection and analysis in the hands of our experts, you can detect proteins expressed on the surface of individual cells and thus identify populations of interest.

Introduction of Flow Cytometry Assay Service

To monitor antigen-specific T cell responses, antigen-specific T cells can be stimulated with MHC-related peptides or antigens to analyze proliferation and cytokine production. Flow cytometry readout can be used for intracellular cytokine staining and is the basis for a variety of assays developed to characterize immune responses by multicolor flow cytometry. It can be combined with cell surface markers for immunophenotypic analysis to measure T cell population sensing.

Like MHC multimer staining, this is a method that allows direct measurement of antigen-specific T cells. However, it has requirements that depend on measuring the same activated cells as the antigen-specific cells. One of its advantages is the co-staining of T cell lineage markers and possibly other surface or intracellular markers (e.g. involved in maturation, suppression or activation), thus increasing the level of knowledge obtained. The combination of flow cytometry and MHC protein staining allows measurement of cytokine expression and provides additional information about the immune response.

Our Flow Cytometry Assay Service

We offer analysis of cell generation by flow cytometry factors including but not limited to effectors and regulatory cytokines in response to stimulation by MHC-associated peptides, antigens or cell lines.

• Analysis of regulatory CD4 or CD8 T cells
• Identification and analysis of immune cell subpopulations
• Detection of HLA activation markers
• Characterization of cytokine staining

Our flow cytometry testing service has a strictly standardized format and also offers our customers the option of individual exchange.

• Available MHC or HLA tissue typing
• Optional MHC- associated custom peptide synthesis

According to your project requirements, we will use optimized solutions to provide you with the best presentation as below.

• MHC immunophenotyping and intracellular cytokine staining were performed according to standardized procedures
• Final assay report, including flow cytometry staining data and raw data files

Advantages of Creative BioMart

• Reliable and robust flow cytometry workflow
• One-stop service for testing, analysis and reporting
• Strict sample quality control
• Competitive pricing within peers
• Reduce your valuable project research time

Turnaround Time

3-5 weeks

Lead times for our MHC-related flow cytometry projects will vary depending on specific requirements, but in general project turnaround times are expected to be 3-5 weeks, including peptide synthesis and assaying of fine samples. We will provide you with the best for MHC-related immunophenotyping or intracellular staining of T cells against other cellular markers or cytokines.

Creative BioMart will work with you to design experiments so that you get clear, usable data and answers to immunological questions related to your MHC. If you are interested in our services, please do not hesitate to contact us for more detailed information.