Creative BioMart offers MHC/peptide affinity analysis services for MHC class I alleles. This service enables rapid turnaround of T cell epitope screening with a custom, personalized solution. Our services target common MHC class I genes to accelerate T cell epitope development.

MHC Class I Molecular Binding Assay

MHC class I molecules are often associated with the presentation of endogenous antigens, and rapid epitope discovery enables the screening of T-cell epitopes of any protein in weeks rather than months or years. MHC class I molecules are present in all vertebrates and control cellular immune responses against viruses and other intracellular pathogens. Complexes are generated when antigenic peptides and MHC class I molecules efficiently interact, which are displayed on the surface of presenting cells and are targeted by peptide-specific, MHCrestricted cytotoxic T cells. If the peptide is of foreign origin, T cells may attack and lyse the target cell. It is important to be able to predict and measure the affinity of peptide-MHC binding. MHC I affinity analysis can significantly speed up the T-cell epitope discovery process and overcome challenges associated with time-consuming, labor-intensive functional assays and limited patient sample sizes. Our services are designed to help you save time and money.

Fig.1 Schematic and highly simplified view of the major histocompatibility complex (MHC) class I presenting pathway. (Claus Lundegaard et al., 2010)

Service Process

Our MHC peptide complex resolution service is used for the analysis of the binding affinity of peptides bound to MHC-I. Although accurate and reliable computational methods have been developed to predict the binding affinity of peptides for binding to MHC-I, these methods have had great success in narrowing the search for T cell epitopes. But its high false positives for immunogenicity (i.e., only a few of the peptides predicted and subsequently shown to bind to MHC-I are actually recognized by T cells after infection) make binding-test-based affinity assays of great potential. The pipeline for this service includes custom peptide synthesis, quantitative binding of candidate peptides to MHC alleles, and binding rate analysis. The MHC peptide binding assay can rapidly determine the ability of each candidate peptide to bind to one or more MHC alleles compared to control peptides.

Advantages of Creative BioMart

• The combination of sub-testing services and multiple testing provides customers with a variety of choices.
• Personalized customized solutions, tailored for each customer.
• Experienced scientists and quality suppliers.

Fig.2 Creative BioMart' featured MHC I affinity analysis service.

Creative BioMart provides high-quality MHC class I peptide complex affinity assays that provide an important evidence component for immunogenicity assessment. Our affinity testing services are part of a binding assay that reflects the binding properties of molecules. The importance of this test is that it can help make up for the shortcomings of screening based on machine computing methods and achieve complementary advantages. If you are interested in our services, please contact us for cooperation.

References

• Lundegaard, Claus, et al. "Major histocompatibility complex class I binding predictions as a tool in epitope discovery." Immunology 130.3 (2010): 309-318.
• Rasmussen, Michael, et al. "Pan-specific prediction of peptide–MHC class I complex stability, a correlate of T cell immunogenicity." The Journal of Immunology 197.4 (2016): 1517-1524.