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| Product Name | PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer |
| Cat. No. | MHCRP-0666 |
| Category | Proteins & Peptides |
| Product Description | Recombinant PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidin. It contains Gly25-Thr305 (HLA-A*03:01) and Ile21-Met119 (B2M). |
| Size | 100/500 μg |
| Format | Liquid. Supplied as 0.22 μm filtered solution in PBS, 300 mM NaCl (pH 7.4). |
| Storage | Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Shipping Temp | Shipped at ambient temperature. |
| Lead Time | Product delivery time depends on the supplier. |
| Species | Human |
| Expression Host | HEK293 |
| Accession No | NP_002107.3(HLA-A*03:01)&P61769(B2M) |
| Tag | C-His-Avi |
| Tag Position | C-terminus |
| Molecular Type | Tetramer |
| Gene Synonyms | MHC; HLA-A*03:01; Peptide Ready |
| Amino Acid Range | Gly25-Thr305 (HLA-A*03:01) & Ile21-Met119 (B2M) |
| Activity | Immobilized PE-labeled human peptide ready HLA-A*03:01&B2M tetramer, His tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA class I (W6/32) antibody, hFc tag with the EC50 of 6.0 ng/mL determined by ELISA. |
| Product Background | Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
| Allele | HLA-A*03:01 |
| Biotinylated | No |
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