Products

PE-Labeled Human HLA-A*02:01&B2M&WT-1 Tetramer

Overview Specification

Product Name	PE-Labeled Human HLA-A*02:01&B2M&WT-1 Tetramer
Cat. No.	MHCRP-0650
Category	Proteins & Peptides
Product Description	Recombinant PE-Labeled Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. PE-Labeled Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl, 0.2% BSA (pH 8.0).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913 (HLA-A*02:01) & P61769 (B2M) & RMFPNAPYL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Tetramer
Gene Synonyms	MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and RMFPNAPYL peptide
Activity	Immobilized PE-labeled human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) tetramer, His tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) antibody, hFc tag with the EC50 of 88.9 ng/mL determined by ELISA.
Product Background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium. The Human HLA-A0201 WT-1 (RMFPNAPYL) complex protein is a complex of HLA-A0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!