Products

Non-biotinylated Human HLA-G Free Heavy Chain Protein

Overview Specification

Product Name	Non-biotinylated Human HLA-G Free Heavy Chain Protein
Cat. No.	MHCRP-0681
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-G Free Heavy Chain Protein is expressed from E. coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (C66S).
Size	100/500 μg
Format	Supplied as 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, 20% Glycerol (pH 8.0).
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	P17693-1
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Weight (MV)	The protein has a predicted MW of 35.5 kDa. The protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Gene Synonyms	HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE.
Amino Acid Range	Gly25-Thr305 (C66S)
Activity	Immobilized human HLA-G free heavy chain, His tag at 2 μg/mL (100 μL/well) on the. Dose response curve for anti-HLA-G antibody, hFc tag with the EC50 of 29.0 ng/mL determined by ELISA (QC Test).
Product Background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Biotinylated	No

For research or industrial raw materials, not for personal medical use!