Products

Mouse H-2K(b)&B2M&OVA Monomer, Ultra Low Endotoxin

Overview Specification

Product Name	Mouse H-2K(b)&B2M&OVA Monomer, Ultra Low Endotoxin
Cat. No.	MHCRP-0634
Category	Proteins & Peptides
Product Description	Recombinant Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Mouse
Expression Host	HEK293
Accession No	P01901(H-2K(b))&P01887(B2M)&SIINFEKL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Gene Synonyms	H-2K; OVA; MHC; H2-K1
Endotoxin Residue	Less than 0.01 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	His24-Pro297(H-2K(b)), Ile21-Met119 (B2M), and SIINFEKL peptide
Product Background	Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. SIINFEKL forms fibrillar assemblies similar to other peptide hydrogels. The immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Allele	H-2K(b)
Biotinylated	No

For research or industrial raw materials, not for personal medical use!