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| Product Name | Human Peptide Ready HLA-A*31:01&B2M Monomer |
| Cat. No. | MHCRP-0629 |
| Category | Proteins & Peptides |
| Product Description | Recombinant Human Peptide Ready HLA-A*31:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*31:01) and Ile21-Met119 (B2M). |
| Size | 100/500 μg |
| Format | Liquid. Supplied as 0.22 μm filtered solution in PBS, 100 mM L-arginine (pH 7.4). |
| Storage | Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Shipping Temp | Shipped at ambient temperature. |
| Lead Time | Product delivery time depends on the supplier. |
| Species | Human |
| Expression Host | HEK293 |
| Accession No | SOQ70041.1(HLA-A*31:01)&P61769(B2M) |
| Tag | C-His-Avi |
| Tag Position | C-terminus |
| Molecular Type | Monomer |
| Molecular Weight (MV) | The protein has a predicted MW of 48.80 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
| Gene Synonyms | MHC; HLA-E*01:03; Peptide Ready |
| Endotoxin Residue | Less than 0.1 EU per μg by the LAL method. |
| Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC. |
| Amino Acid Range | Gly25-Thr305 (HLA-A*31:01) & Ile21-Met119 (B2M) |
| Product Background | HLA-A*31:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*31:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
| Allele | HLA-A*31:01 |
| Biotinylated | No |
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