Products

Human HLA-G&B2M&Peptide Tetramer

Overview Specification

Product Name	Human HLA-G&B2M&Peptide Tetramer
Cat. No.	MHCRP-0672
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Tetramer
Molecular Weight (MV)	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Gene Synonyms	HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide
Activity	Immobilized human HLA-G&B2M&peptide (RIIPRHLQL) tetramer, His tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA-G antibody, hFc tag with the EC50 of 6.2 ng/mL determined by ELISA (QC Test). The affinity constant of 4.62 nM as determined in SPR assay.
Product Background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Allele	HLA-G
Biotinylated	No

For research or industrial raw materials, not for personal medical use!