Products

Human HLA-E*01:03&B2M&Peptide Monomer Negative Control

Overview Specification

Product Name	Human HLA-E*01:03&B2M&Peptide Monomer Negative Control
Cat. No.	MHCRP-0622
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-E01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein is expressed from HEK293 with His tag and Avi at the C-terminus. It contains Gly22-Thr302 (HLA-E01:03), Ile21-Met119 (B2M) and VMAPKTLVL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	P13747(HLA-E*01:03)&P61769(B2M)&VMAPKTLVL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Gene Synonyms	HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC; QA1
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly22-Thr302 (HLA-E*01:03), Ile21-Met119 (B2M), and VMAPKTLVL peptide
Product Background	HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Allele	HLA-E*01:03
Biotinylated	No

For research or industrial raw materials, not for personal medical use!