Products

Human HLA-B*15:01&B2M&SARS-CoV-2 Epitope Monomer

Overview Specification

Product Name	Human HLA-B*15:01&B2M&SARS-CoV-2 Epitope Monomer
Cat. No.	MHCRP-0618
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-B15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly21-Thr301 (HLA-B15:01), Ile21-Met119 (B2M) and NQKLIANQF peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	AAA53258.1(HLA-B*15:01)&P61769(B2M)&NQKLIANQF
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MHC; SARS-CoV-2 epitope
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly21-Thr301 (HLA-B*15:01), Ile21-Met119 (B2M), and NQKLIANQF peptide
Product Background	HLA-B15:01 is strongly associated with asymptomatic infection with SARS-CoV-2 and is likely to be involved in the mechanism underlying early viral clearance. T cells from pre-pandemic individuals carrying HLA-B15:01 were reactive to the immunodominant SARS-CoV-2 S-derived peptide NQKLIANQF, and 100% of the reactive cells displayed memory phenotype.
Allele	HLA-B*15:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!