Products

Human HLA-A*03:01&B2M&GP100 (YLEPGPVTA) Tetramer

Overview Specification

Product Name	Human HLA-A*03:01&B2M&GP100 (YLEPGPVTA) Tetramer
Cat. No.	MHCRP-0490
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-A02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and YLEPGPVTA peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Tetramer
Molecular Weight (MV)	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Gene Synonyms	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and YLEPGPVTA peptide
Activity	Immobilized human HLA-A02:01&B2M&GP100 (YLEPGPVTA) tetramer, His tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA-A02:01&B2M&GP100 (YLEPGPVTA) antibody, hFc tag with the EC50 of 5.6 ng/mL determined by ELISA (QC Test). The affinity constant of 0.196 nM as determined in SPR assay.
Product Background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!