Products

Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer

Overview Specification

Product Name	Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer
Cat. No.	MHCRP-0548
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-A02:01&B2M&TdT (ALYDKTKRI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and ALYDKTKRI peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&ALYDKTKRI
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	DNA nucleotidylexotransferase; terminal deoxynucleotidyl transferase
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and ALYDKTKRI peptide
Activity	Immobilized human HLA-A*02:01&B2M&TdT (ALYDKTKRI) monomer, His tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 11.3 ng/mL determined by ELISA.
Product Background	The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!