Products

Human HLA-A*02:01&B2M&P53 WT Monomer, HEK293

Overview Specification

Product Name	Human HLA-A*02:01&B2M&P53 WT Monomer, HEK293
Cat. No.	MHCRP-0536
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-A02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and HMTEVVRRC peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRRC
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and HMTEVVRRC peptide
Activity	Immobilized human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) monomer, His tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 8.1 ng/mL determined by ELISA (QC Test).
Product Background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein. p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!