Products

Human HLA-A*02:01&B2M&CMV pp65 Tetramer, E. coli

Overview Specification

Product Name	Human HLA-A*02:01&B2M&CMV pp65 Tetramer, E. coli
Cat. No.	MHCRP-0485
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-A02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Tetramer
Molecular Weight (MV)	The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Bis-Tris PAGE result under Non reduced (N) condition.
Gene Synonyms	CMVpp65; NLVPMVATV
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and NLVPMVATV peptide
Product Background	Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!