Products

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, Lyophilized

Overview Specification

Product Name	Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, Lyophilized
Cat. No.	MHCRP-0477
Category	Proteins & Peptides
Product Description	Recombinant Human HLA-A02:01&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and FMNKFIYEI peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Gene Synonyms	Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and FMNKFIYEI peptide
Activity	Immobilized human HLA-A02:01&B2M&AFP (FMNKFIYEI) monomer, His tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA-A02:01&B2M&AFP (FMNKFIYEI) antibody, hFc tag with the EC50 of 1.9 ng/mL determined by ELISA (QC Test). The affinity constant of 0.923 μM as determined in SPR assay.
Product Background	Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins. CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!