Products

Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer

Overview Specification

Product Name	Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer
Cat. No.	MHCRP-0468
Category	Proteins & Peptides
Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and ILAKFLHWL peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&ILAKFLHWL
Tag	C-His
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	TERT; ILAKFLHWL
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M), and ILAKFLHWL peptide
Product Background	The human telomerase catalytic subunit human telomerase reverse transcriptase (hTERT) is expressed in many human cancer cells derived from multiple tissues, but infrequently in normal cells. Thus, hTERT is an attractive candidate target for tumor immunotherapy. hTERT:540–548 peptide (p540, ILAKFLHWL) has been identified as an effective HLA-A*0201-restricted T-cell epitope, an effective T-cell-based cancer treatment, including vaccines, against hTERT will likely require the identification of other MHC class I-restricted epitopes in this antigen.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!