Products

Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer

Overview Specification

Product Name	Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer
Cat. No.	MHCRP-0467
Category	Proteins & Peptides
Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and HMTEVVRHC peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human&Mouse
Expression Host	HEK293
Accession No	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&HMTEVVRHC
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Purity	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC.
Amino Acid Range	Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M), and HMTEVVRHC peptide
Activity	Immobilized anti-HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) antibody, hFc Tag, hFc tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for chimeric HLA-A02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) monomer, His tag with the EC50 of 0.47 μg/mL determined by ELISA.
Product Background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein. p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!