Products

Chimeric HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer

Overview Specification

Product Name	Chimeric HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer
Cat. No.	MHCRP-0466
Category	Proteins & Peptides
Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and SLLMWITQC peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human&Mouse
Expression Host	HEK293
Accession No	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&SLLMWITQC
Tag	C-His
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 48.02 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A
Purity	> 95% as determined by Bis-Tris PAGE.
Amino Acid Range	Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M), and SLLMWITQC peptide
Activity	Immobilized anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) antibody, hFc tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for chimeric HLA-A02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) monomer, His tag with the EC50 of 23.0 ng/mL determined by ELISA (QC Test). The affinity constant of 9.32 nM as determined in SPR assay.
Product Background	NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!