Products

Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer

Overview Specification

Product Name	Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer
Cat. No.	MHCRP-0465
Category	Proteins & Peptides
Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and KVLEHVVRV peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human&Mouse
Expression Host	HEK293
Accession No	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&KVLEHVVRV
Tag	C-His
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MAGE-A4; MAGE-A8
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M), and KVLEHVVRV peptide
Activity	The affinity constant of 4.76 μM as determined in SPR assay.
Product Background	MAGE-A4 and MAGE-A8 are type I members of the melanoma associated antigen (MAGE) family. The MAGE family is a large, highly conserved group of proteins that share a common MAGE homology domain. Both MAGE-A4 and MAGE-A8 antigen-presenting peptides can be presented by HLA-A*02:01.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!