Products

Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 Monomer

Overview Specification

Product Name	Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 Monomer
Cat. No.	MHCRP-0461
Category	Proteins & Peptides
Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human&Mouse
Expression Host	HEK293
Accession No	A0A140T933(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&YLEPGPVTA
Tag	C-His
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 47.90 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M), and YLEPGPVTA peptide
Activity	Immobilized chimeric HLA-A02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) monomer, His tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA-A02:01&B2M&GP100 (YLEPGPVTA) antibody, hFc tag with the EC50 of 25.3 ng/mL determined by ELISA (QC Test). The affinity constant of 67.84 nM as determined in SPR assay.
Product Background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!