Products

Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer

Overview Specification

Product Name	Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer
Cat. No.	MHCRP-0460
Category	Proteins & Peptides
Product Description	Recombinant Chimeric HLA-A02:01(mα3)&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr206(Human HLA-A02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human&Mouse
Expression Host	HEK293
Accession No	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P61769(B2M)&RMFPNAPYL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Tetramer
Molecular Weight (MV)	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Gene Synonyms	MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (B2M), and RMFPNAPYL peptide
Activity	Immobilized chimeric HLA-A02:01 (mα3)&B2M&WT-1 (RMFPNAPYL) tetramer, His tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) antibody, hFc tag with the EC50 of 6.3 ng/mL determined by ELISA.
Product Background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium. The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!