Products

Biotinylated Rhesus macaque HLA-G&B2M&Peptide Monomer Protein

Overview Specification

Product Name	Biotinylated Rhesus macaque HLA-G&B2M&Peptide Monomer Protein
Cat. No.	MHCRP-0680
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Rhesus macaque
Expression Host	HEK293
Accession No	O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide
Activity	Immobilized biotinylated rhesus macaque HLA-G&B2M&peptide (RIIPRHLQL), His tag at 2 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for rhesus macaque LILRB1, hFc tag with the EC50 of 0.18 μg/mL determined by ELISA.
Product Background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!