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| Product Name | Biotinylated Mouse I-Ab&OVA 323-339 Monomer |
| Cat. No. | MHCRP-0454 |
| Category | Proteins & Peptides |
| Product Description | Recombinant Biotinylated Mouse I-Ab&OVA 323-339 (ISQAVHAAHAEINEAGR) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Ile27-Glu218 (H2-Aa), Ser30-Lys226 (H2-Ab1) and ISQAVHAAHAEINEAGR peptide. |
| Size | 100/500 μg |
| Format | Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4). |
| Storage | Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Shipping Temp | Shipped with dry ice. |
| Lead Time | Product delivery time depends on the supplier. |
| Species | Mouse |
| Expression Host | HEK293 |
| Accession No | P14434(H2-Aa)&P14483(H2-Ab1)&ISQAVHAAHAEINEAGR |
| Tag | C-His-Avi |
| Tag Position | C-terminus |
| Molecular Type | Monomer |
| Molecular Weight (MV) | The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa and 42-50 kDa based on Bis-Tris PAGE result. |
| Gene Synonyms | I-Ab&OVA 323-339 (ISQAVHAAHAEINEAGR) |
| Endotoxin Residue | Less than 1 EU per μg by the LAL method. |
| Purity | > 95% as determined by Bis-Tris PAGE. |
| Amino Acid Range | Ile27-Glu218 (H2-Aa), Ser30-Lys226 (H2-Ab1), and ISQAVHAAHAEINEAGR peptide |
| Product Background | OVA 323–339 (ISQAVHAAHAEINEAGR) is a 17–amino acid peptide from chicken Ovalbumin. It is a well-known CD4+ T-cell epitope presented by MHC class II molecules to activate helper T cells. This peptide is widely used in mice to study antigen-specific T-cell responses and immune regulation, including models of allergy and autoimmunity. Its defined sequence and reproducible immunogenicity make it a common tool in immunology research. |
| Allele | I-Ab |
| Biotinylated | Yes |
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