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| Product Name | Biotinylated Mouse H-2K(b)&B2M&OVA Monomer |
| Cat. No. | MHCRP-0453 |
| Category | Proteins & Peptides |
| Product Description | Recombinant Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide. |
| Size | 100/500 μg |
| Format | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage | Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Shipping Temp | Shipped with dry ice. |
| Lead Time | Product delivery time depends on the supplier. |
| Species | Mouse |
| Expression Host | HEK293 |
| Accession No | P01901(H-2K(b))&P01887(B2M)&SIINFEKL |
| Tag | C-His-Avi |
| Tag Position | C-terminus |
| Molecular Type | Monomer |
| Gene Synonyms | H-2K; OVA; MHC; H2-K1 |
| Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC. |
| Amino Acid Range | His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M), and SIINFEKL peptide |
| Product Background | Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. SIINFEKL forms fibrillar assemblies similar to other peptide hydrogels. The immunoactive properties of this peptide can therefore be related to its self-assembling nature. |
| Biotinylated | Yes |
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