Products

Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer

Overview Specification

Product Name	Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer
Cat. No.	MHCRP-0444
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human Peptide Ready HLA-A33:03&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A33:03) and Ile21-Met119 (B2M).
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS, 100 mM L-arginine (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	VCU43110.1(HLA-A*33:03)&P61769(B2M)
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MHC; HLA-A*33:03; Peptide Ready
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*33:03) & Ile21-Met119 (B2M)
Activity	Immobilized biotinylated human peptide ready HLA-A*33:03&B2M monomer, His tag at 0.5 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-HLA class I (W6/32) antibody, hFc tag with the EC50 of 3.5 ng/mL determined by ELISA.
Product Background	HLA-A33:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A33:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Allele	HLA-A*33:03
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!