Products

Biotinylated Human HLA-E*01:03&B2M&Peptide Monomer

Overview Specification

Product Name	Biotinylated Human HLA-E*01:03&B2M&Peptide Monomer
Cat. No.	MHCRP-0435
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-E01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly22-Thr302 (HLA-E01:03), Ile21-Met119 (B2M) and VMAPRTLVL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Gene Synonyms	HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly22-Thr302 (HLA-E*01:03), Ile21-Met119 (B2M), and VMAPRTLVL peptide
Activity	Immobilized biotinylated human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) monomer, His tag at 0.5 μg/mL (100 μL/well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 5.7 ng/mL determined by ELISA (QC Test).
Product Background	HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Allele	HLA-E*01:03
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!