Products

Biotinylated Human HLA-A*11:01&B2M&EGFR L858R Monomer, E. coli

Overview Specification

Product Name	Biotinylated Human HLA-A*11:01&B2M&EGFR L858R Monomer, E. coli
Cat. No.	MHCRP-0399
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and KITDFGRAK peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	AAV53343.1(HLA-A*11:01)&P61769(B2M)&KITDFGRAK
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Gene Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M), and KITDFGRAK peptide
Activity	Immobilized biotinylated HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) monomer, His-Avi tag at 2 μg/mL (100 μL/well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for HLA-A11:01&B2M&EGFR TCR, hFc tag with the EC50 of 1.6 ng/mL determined by ELISA (QC Test).
Product Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Allele	HLA-A*11:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!