Products

Biotinylated Human HLA-A*03:01&B2M&IGA2 Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*03:01&B2M&IGA2 Monomer
Cat. No.	MHCRP-0390
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M) and FTWTPSSGK peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	NP_002107.3(HLA-A*03:01)&P61769(B2M)&FTWTPSSGK
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 35.26 kDa (HLA-A*03:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Gene Synonyms	MHC; IGA2
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M), and FTWTPSSGK peptide
Activity	Immobilized biotinylated human HLA*03:01&B2M&IGA2 (FTWTPSSGK) monomer, His tag at 0.5 μg/mL (100 μL/well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 8.0 ng/mL determined by ELISA.
Product Background	The immunoglobulin variable region is a part of an antibody molecule responsible for antigen recognition and binding. It is located at the N-terminal end of each antibody chain and is highly diverse due to the presence of variable (V), diversity (D), and joining (J) gene segments in B cells that undergo somatic recombination during antibody production. This variability allows the immune system to recognize a wide range of antigens.
Allele	HLA-A*03:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!