Products

Biotinylated Human HLA-A*02:01&B2M&Survivin (LTLGEFLKL) Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&Survivin (LTLGEFLKL) Monomer
Cat. No.	MHCRP-0383
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&Survivin (LTLGEFLKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and LTLGEFLKL peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.44 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	Survivin; MHC; MHC I; BIRC5; API4; EPR-1; IAP4; survivin variant 3 alpha
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and LTLGEFLKL peptide
Activity	Immobilized biotinylated human HLA-A*02:01&B2M&Survivin (LTLGEFLKL) monomer, His tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 10.9 ng/mL determined by ELISA (QC Test).
Product Background	Survivin (also known as BIRC5) is an evolutionarily conserved eukaryotic protein that is essential for cell division and can inhibit cell death. Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!