Products

Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer
Cat. No.	MHCRP-0382
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&Survivin (LMLGEFLKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01),Ile21-Met119 (B2M) and LMLGEFLKL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&LMLGEFLKL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result.
Gene Synonyms	Survivin; MHC; MHC I; BIRC5; API4; EPR-1; IAP4; survivin variant 3 alpha
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and LMLGEFLKL peptide
Activity	Immobilized biotinylated human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) monomer, His tag at 1 μg/mL (100 μL/well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 13.6 ng/mL determined by ELISA (QC Test).
Product Background	Survivin (also known as BIRC5) is an evolutionarily conserved eukaryotic protein that is essential for cell division and can inhibit cell death. Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!