Products

Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer
Cat. No.	MHCRP-0378
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and ALYVDSLFFL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	PRAME; OIP-4; OIP4; MAPE
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and ALYVDSLFFL peptide
Activity	Immobilized biotinylated human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) monomer, His tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-HLA-A02:01&B2M&PRAME (ALYVDSLFFL) antibody, hFc tag with the EC50 of 6.2 ng/mL determined by ELISA.
Product Background	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!