Products

Biotinylated Human HLA-A*02:01&B2M&P53 R175H Monomer, E. coli

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&P53 R175H Monomer, E. coli
Cat. No.	MHCRP-0373
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Gene Synonyms	MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and HMTEVVRHC peptide
Activity	Immobilized biotinylated human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) monomer, His tag at 2 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) antibody, hFc tag with the EC50 of 4.4 ng/mL determined by ELISA (QC Test).
Product Background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein. p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!