Products

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 Monomer, HEK293

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 Monomer, HEK293
Cat. No.	MHCRP-0362
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and GVYDGREHTV peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Gene Synonyms	HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and GVYDGREHTV peptide
Activity	Immobilized biotinylated human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) monomer, His tag at 0.5 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) antibody, hFc tag with the EC50 of 3.0 ng/mL determined by ELISA.
Product Background	Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene. The MAGE-A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A0201 of -the MHC Class I, B2M and GVYDGREHTV peptide of -the MAGE-A4.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!