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| Product Name | Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Monomer |
| Cat. No. | MHCRP-0363 |
| Category | Proteins & Peptides |
| Product Description | Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVLEHVVRV peptide. |
| Size | 100/500 μg |
| Format | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage | Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Shipping Temp | Shipped at ambient temperature. |
| Lead Time | Product delivery time depends on the supplier. |
| Species | Human |
| Expression Host | HEK293 |
| Accession No | A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEHVVRV |
| Tag | C-His-Avi |
| Tag Position | C-terminus |
| Molecular Type | Monomer |
| Molecular Weight (MV) | The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result. |
| Gene Synonyms | MAGE-A4; MAGE-A8 |
| Endotoxin Residue | Less than 1 EU per μg by the LAL method. |
| Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC. |
| Amino Acid Range | Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and KVLEHVVRV peptide |
| Product Background | MAGE-A4 and MAGE-A8 are type I members of the melanoma associated antigen (MAGE) family. The MAGE family is a large, highly conserved group of proteins that share a common MAGE homology domain. Both MAGE-A4 and MAGE-A8 antigen-presenting peptides can be presented by HLA-A*02:01. |
| Allele | HLA-A*02:01 |
| Biotinylated | Yes |
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